Fig. 5.

Palmitic acid does not trigger extracellular nucleosome production in cultured human hepatoma cells or adipocytes. a HepG2 cells were incubated for 24 h with palmitic acid at the indicated concentrations, before nucleosome absorbance was measured in the extracellular medium. b Human multipotent adipose-derived stem (hMADS) cells and pre-adipocytes from Simpson-Golabi-Behmel congenital syndrome (SGBS) were cultured and samples from cell culture supernatants were collected at 3, 5, 8, 10, and 12 days after adipocyte differentiation (see text), for subsequent measuring nucleosome concentration. The data represent the means ± SEM of three independent experiments