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. 2019 Dec 22;25:9836–9843. doi: 10.12659/MSM.918405

Figure 4.

Figure 4

MiR-26a-5p inhibits ox-LDL-induced migration and the release of adhesion-related molecules by regulating PTEN. (A) Predicted consequential pairing of PTEN and miR-26a-5p. (B) qRT-PCR was applied to detect the expression of PTEN in HUVECs after transfection with miR-26a-5p mimic, miR-26a-5p inhibitor and NC; n=6. ** P<0.01, *** P<0.001 compared to negative control (NC). (C) Western blot was applied to detect the expression of PTEN in HUVECs after transfection with miR-26a-5p mimic, miR-26a-5p inhibitor, and NC; n=6. *** P<0.001 compared to NC. (D, E) qRT-PCR was applied to detect the expression of PTEN in ox-LDL-induced HUVECs after being transfected with pc-DNA, pc-PTEN, sh-NC, and sh-PTEN. (F) Relative migration ratio. (G, H) qRT-PCR was applied to detect the expression of MCP-1 and ICAM-1 in ox-LDL-induced HUVECs after being transfected with pc-DNA, pc-PTEN, sh-NC, and sh-PTEN; n=4. *** P<0.001 compared to the indicated group. ox-LDL – oxidized low-density lipoprotein; PTEN – phosphatase and tensin homolog; HUVECs – human umbilical vascular endothelial cells; qRT-PCR – quantitative real-time polymerase chain reaction; CTL – control.