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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Dev Biol. 2019 Sep 14;457(1):43–56. doi: 10.1016/j.ydbio.2019.09.003

Fig. 1. SOX2 is eliminated in the ear and pre-lens placodes in early Sox2cKO embryos.

Fig. 1.

Whole mount analysis of SOX2 expression pattern in E8.5 control, heterozygous Foxg1-Cre;Sox2+/f, and Sox2cKO embryos (11 somites), displayed in a dorsal view (control n = 9, Sox2cKO n = 5, Foxg1-Cre;Sox2+/f n = 5). Foxg1-Cre expression is visualized by using anti-Cre antibody in heterozygous Foxg1-Cre;Sox2+/f, and Sox2cKO embryos (C-F). Split channel images show SOX2 (C’-F’) and Cre immunolabeling (C”-F”). The dotted oval indicates the area of the otic pit (A, C-C”, D-D”). SOX2 expression is lost in the Sox2cKO ear invaginating placode (D, D’) compared to control (A), and heterozygous Foxg1-Cre;Sox2+/f embryos (C, C’). In the developing eye, SOX2 is eliminated in the presumptive lens ectoderm (PLE) and decreased SOX2 levels are evident in many cells of the optic vesicle (OV) in Sox2cKO (F, F’) compared to control embryos (B) and Foxg1-Cre;Sox2+/f (E, E’). Note Foxg1-Cre expression in the surface ectoderm in the area of the PLE (E, F, E”, F”). HS, Hoechst nuclear staining. Scale bars: 50 μm.