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. 2020 Jan 1;34(1-2):37–52. doi: 10.1101/gad.329110.119

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© 2020 Robles-Murguia et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — Increase in ple/TH expression in dopaminergic neurons promotes foraging. (A–C) Monitoring of the spontaneous locomotion of flies with general ple/TH overexpression in dopaminergic neurons. Three monitors are located at different distance from the food. In addition to increasing overall activity (B,C), overexpression of ple/TH with TH-Gal4 promotes locomotion nearby the food (monitor #1) (A,C) compared with control GFP overexpression. Mean values are shown in A and B, and the corresponding bar graphs with mean ± SD are shown in C, with n[TH > GFP] = 6 and n[TH > ple] = 6 batches of 15 flies each. (D) ple/TH overexpression with TH-Gal4 promotes feeding initiation, as assessed with PER assays, with n[TH > GFP] = 258 and n[TH > ple] = 299; P < 0.05, 95% CI. (E–G) ple/TH overexpression in subsets of dopaminergic neurons with TH-C-Gal4 promotes spontaneous locomotion in proximity of food (monitor #1) (E,G), whereas this does not occur with TH-D-Gal4 (I,K), which drives expression in a different subset of dopaminergic neurons. (F,G,J,K) ple/TH overexpression with both TH-C-Gal4 and TH-D-Gal4 increases overall activity. (H,L) ple/TH overexpression with TH-C-Gal4 but not with TH-D-Gal4 promotes feeding initiation, as estimated with PER assays, with n[TH-C > GFP] = 185, n[TH-C > ple] = 169, n[TH-D > GFP] = 203, and n[TH-D > ple] = 180. P < 0.05; 95% CI. Mean values are shown in E, F, I, and J), and the corresponding bar graphs with mean ± SD are shown in G and K, with n[TH-C > GFP] = 5, n[TH-C > ple] = 4, n[TH-D > GFP] = 5, and n[TH-D > ple] = 4 batches of 15 flies each.