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. 2020 Jan 1;34(1-2):87–98. doi: 10.1101/gad.331868.119

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© 2020 Shyian et al.; Published by Cold Spring Harbor Laboratory Press

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Figure 5. — Fork pausing is a separable function of Tof1–Csm3. (A, C, D) Western blotting of TCA-extracted proteins. (A) In contrast to tof1Δ, tof1-ΔC cells do not degrade Csm3-TAP. (B,E) Serial dilution growth assays. (B) Tof1-ΔC supports viability of rad9Δ cells under hydroxyurea (HU) treatment. (C,D) Tof1-ΔC is proficient in DRC activation under HU treatment. (E) Mrc1 supports tof1-ΔC cells survival under topoisomerase-blocking damage. (CPT) Camptothecin; (ETOP) etoposide; (MMS) methyl methanesulfonate; (12geneΔ0HSR) multidrug sensitive yeast background. See also Supplemental Figure S5.