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. Author manuscript; available in PMC: 2020 May 13.
Published in final edited form as: J Vis Exp. 2019 May 13;(147):10.3791/59342. doi: 10.3791/59342

Figure 1: Generation of iPSC-EPs from Pluripotent Stem Cells.

Figure 1:

A. WiCell 19-9-11 iPSCs, which stained positive for Oct4, were cultured in E8 medium supplemented with 10 μM Y-27632 ROCK inhibitor for 48 hours. B. The iPSCs were then induced with 6 μM of CHIR99021 in LaSR Basal medium for 48 hours, at which point the cells were positive for Brachyury, a mesoderm marker. C. The cells were further cultured in LaSR Basal media until they expressed CD34, a marker for endothelial progenitors. D. Roughly 15–25% of the differentiated cells expressed CD34. All scale bars represent lengths of 200 μm.