(A) H&E stained lung sections (scale bar=100mm), (B) eosinophils, and (C) IL-5 and IL-13 from PBS or Alternaria (Alt) challenged mice. Identification, frequency (D), and total numbers (E) of ILCs. Ki67+ frequency within ILC subpopulations (E). Frequency and total number of IL-5+ and GATA-3+ ILCs (F & G). % IL-5+ ILC2s gated from all Thy1.2+ ILCs (H) and ILC ST2/CD127 gating from all IL-5+ ILCs (I) from Red5 mice. Data shown are representative of two to nine independent experiments with three mice per group. *p < 0.05, **p < 0.01, ***p < 0.001, unpaired t test.