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. 2020 Jan 1;6(1):eaax6969. doi: 10.1126/sciadv.aax6969

Fig. 5. Characterizing energetics of − 1 frameshifting in vivo.

Fig. 5

(A) Left: Cartoon energy landscapes of frameshifting on the slippery sequence at different temperatures. Right: The −1 frameshifting efficiency measured on the slippery sequence without mRNA structure at different temperatures. (B) Left: Cartoon energy landscapes of the mRNA structure unfolding before translocation at different temperatures. Right: The −1 frameshifting efficiency measured on the slippery sequence accompanied by the downstream mRNA structure at different temperatures. (C) Top: Frameshifting cassettes composed of tandem repeats of slippery sequences. Bottom left: Frameshifting efficiency of constructs with different repeat numbers measured at 35°C. Bottom right: Frameshifting efficiency of the mRNA construct with five slippery sequence repeats measured at different temperatures (means ± SD are shown from n = 3 technical replicates for all plots in this figure; values are summarized in tables S1 and S2; gel images are present in fig. S5).