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. 2019 Dec;16(12):855–871. doi: 10.11909/j.issn.1671-5411.2019.12.003

Figure 4. The mitochondrial biogenesis and function under hypoxia is dependent on HIF-1α in vivo and in vitro.

Figure 4.

(A): The relative mitochondrial DNA copy number was determined by comparison to nuclear DNA (rRNA 18S); (B): ATPs activity relative was measured by using spectrophotometer in different conditions; (C): complex IV activity relative was measured by using spectrophotometer in different conditions; (D): the ROS was detected by using fluorescent dye dichlorohydrofluorescein diacetate assay (magnification, 400 ×); (E): measurement of ROS generation in different conditions; (F): MMP as determined using the JC-1 assay (magnification, 400 ×); and (G): MMP as determined using the JC-1 assay kit in different conditions. ATPs: adenosine triphosphates; DAPI: 4,6-diamond-2-phenyl insole; HIF-1α: hypoxia-inducible factor-1α; MMP: mitochondrial membrane potential; ROS: reactive oxygen species; siHIF-1α: small interfering hypoxia-inducible factor-1α.