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. 2019 Nov 19;11(1):82–94. doi: 10.1111/1759-7714.13236

Figure 5.

Figure 5

Identification of miR‐148a‐3p targeting MCL‐1. (a) The putative binding site of miR‐148a‐3p and MCL‐1. (b.c) The target relationship was identified by dual‐luciferase reporter assay. Mimic‐NC and miR‐148a‐3p mimic were designed to explore the effect of MCL‐1 by loss‐and‐gain functional experiment on ESCC cells. (Inline graphic) mimic control and (Inline graphic) miR‐148a‐3p mimic. (d,e) QRT‐PCR was used to detect miR‐148a‐3p level in each group. (f,g) Western blot was used to detect miR‐148a‐3p level in each group. *P < 0.05.