Fig. 1.

High expression of hPG80 by tumor cells from colorectal cancer. (A) Confocal image of a tumoral mass showing high expression of hPG80 in colon cancer (patient 6607). The tumor cells expressing hPG80 were visualized with IHC for hPG80 (red). The hPG80-immunoreactive cells were often clustered into masses (arrow) into low-differentiated glands surrounded by disrupted basement membranes (arrowhead, HSPG-immunoreactivity, green). The double arrowhead indicates autofluorescence in the connective tissue (blue), which contrasts with cell nuclear counterstaining with bisbenzimide (blue, double arrow). (B) The tissue peripheral to the tumor mass showed scarce hPG80-immunoreactive cells in the connective tissue (arrow) and linear basement membranes (arrowhead). (C) Magnified field of the area indicated by an arrowhead in (A) showing the perinuclear location of hPG80-immunoreactivity (arrows). (D) Magnified field of the peripheral tissue showing the linear basement membrane (arrow), nuclei of epithelial cells (arrowhead) and the lumen of the gland (double arrow). (E) Highly-magnified field showing one cell and the cytoplasmic localization of hPG80. (F) Absence of hPG80-immunoreactive cells in the peripheral tissue. Scale bars: A and B: 50 µm; C and D: 20 µm; E and F: 5 µm.