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. 2019 Dec 20;30:101411. doi: 10.1016/j.redox.2019.101411

Fig. 2.

Fig. 2

Effects of salusin-β knockdown on cisplatin-induced renal tubular cell damage. HK-2 cells were transfected with adenovirus mediated shRNA against salusin-β (MOI = 100) for 48 h, and then used for cisplatin (20 μM) stimulation for 24 h. (A) Relative mRNA levels of KIM-1 and NGAL. (B) Caspase-3 activity. (C) Cell apoptosis determined with TUNEL assay. Blue fluorescence (Hoechst 33342) shows cell nuclei and green fluorescence (TUNEL) stands for apoptotic cells. (D) The ratio of TUNEL-positive cells to total cells. (E) Representative blots and quantitative analysis of Bcl-2. (F) Representative blots and quantitative analysis of Bax. (G) Representative blots and quantitative analysis of cleaved-caspase-3 (C-caspase-3). (H) Representative blots and quantitative analysis of cleaved-PARP (C-PARP). (I) Representative blots and quantitative analysis of γH2AX at 24 h after cisplatin (20 μM) stimulation. (J) Representative blots and quantitative analysis of total and phosphorylated p53. Scale bar = 50 μm. Values are mean ± SE. *P < 0.05 vs. Control, †P < 0.05 vs. Cisplatin. n = 6 for each group. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)