Skip to main content
. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Immunol Methods. 2019 Oct 18;476:112686. doi: 10.1016/j.jim.2019.112686

Figure 1: Flow cytometry reliably detects DAT and TH expression in human PBMCs obtained from whole blood.

Figure 1:

Representative plots showing the gating strategy for DAT- and TH-expressing human monocytes by flow cytometry. (A) doublet discrimination and debris exclusion; (B) DAT and TH expressing monocytes can be discriminated based on sidescatter properties intrinsic to monocytes (B, 1st and 2nd panel from left) or by expression of human monocyte marker CD14 (B, 3rd and 4th panel from left). (C) Isotype controls reveal minimal nonspecific staining. (D) Histogram representations for anti-DAT, anti-TH and anti-CD14 displaying mean fluorescence intensity (MFI) for: unstained (black), isotype control (blue) and immune-stained (red). N= 41 independent biological replicates