Fig. 1.

The composition of Leishmania glycans within PSG from the permissive vector Lu. longipalpis. Western Blot analysis of L. major, L. tropica and L. mexicana PSG probed with LT15, a mAb which recognizes the Gal-P-Man disaccharide backbone of all Leishmania phosphoglycans (Ilg et al., 1996). Each lane represents the equivalent of one sand fly's PSG extracted from mature infections (L. major and L. tropica, day 12–13 p.i.; L. mexicana day 8 p.i.). The last lane represents the luminal content of one uninfected sand fly midgut probed with LT15. Arrow indicates a junction between stacking and resolving gel. MWM, molecular weight markers.