Figure EV1. Single‐cell RNA‐seq‐based reconstruction of monoamine signaling in human pancreatic β cells.

• A, B
  Single‐cell RNA‐seq analysis of β (A‐A₃) and α cells (B‐B₃) from healthy (n = 6) and type 2 diabetic (n = 4) donors revealed the presence (or lack) of molecular determinants for serotonin, epinephrine/norepinephrine, and dopamine signaling. Note particular differences in serotonin transporter (SLC6A4), tryptophan hydroxylase (TPH2), and tyrosine hydroxylase (TH) expression between β and α cells. Expression of the vesicular monoamine transporter (VMAT2), which is non‐selective for monoamines, was plotted separately (A₃, B₃). Data were expressed as log₂ reads per kilobase of transcript per million mapped reads (RPKM). Data were re‐processed from an open‐source database (Segerstolpe et al, 2016).