Figure EV4. Consequences of intrauterine amphetamine exposure on select transcription factors at birth and in Scl6a4 ^−/− mice.

1. No significant difference was noted in the number of histochemically detectable Pdx1⁺ cells prenatally exposed to amphetamine versus vehicle‐treated controls at P0. An effect of sex was not observed either.
2. Real‐time PCR experiments pointed to a sex‐independent reduction of Ins1, NeuroD1, and Nkx6.1 mRNAs in pancreata from P0 mice. Note that amphetamine in all cases induced a marked reduction albeit reaching statistical significance (n = 3 animals/sex/group).
3. In female offspring, Slc6a4 knock‐out seemed to phenocopy the effect of intrauterine amphetamine exposure by significantly reducing the number of insulin⁺ β cells (C1). Note that α cells were also adversely affected in this experiment. n ≥ 3 animals/sex/group.

Data information: Scale bar = 40 μm (C), 25 μm (A). Data were expressed as means ± SEM. **P < 0.01, *P < 0.05 (pair‐wise comparisons after one‐way ANOVA).