Table 1.
Primer sequences used for quantitative analysis in this study
| Gene | Primer sequence | Species |
|---|---|---|
| Gapdh | Forward: 5′‐AACTTTGGCATTGTG‐3′ | Mouse |
| Reverse: 5′‐ACACATTGGTAG‐3′ | ||
| Gcg | Forward: 5′‐TACACCTGTTCGCAGCTCAG‐3′ | Mouse |
| Reverse: 5′‐CTCTGTGTCTTGAAGGGCGT‐3′ | ||
| Gcg | Forward: 5′‐AGGAACCGGAACAACATTGC‐3′ | Rat |
| Reverse: 5′‐CCTTTCACCAGCCAAGCAAT‐3′ | ||
| Htr2b | Forward: 5′‐TGTTTGTGATGCCGATTGCC‐3′ | Mouse |
| Reverse: 5′‐AGTTGCACTGATTGGCCTGA‐3′ | ||
| Ins1 | Forward: 5′‐CCACCCAGGCTTTTGTCAAA‐3′ | Mouse |
| Reverse: 5′‐CCAGTTGTTCCACTTGTGGG‐3′ | ||
| Ins1 | Forward: 5′‐GTGGGGAACGTGGTTTCTTC‐3′ | Rat |
| Reverse: 5′‐TGCAGCACTGATCCACAATG‐3′ | ||
| Ins2 | Forward: 5′‐CCACAAGTGGCACAACTGGA‐3′ | Mouse |
| Reverse: 5′‐CTACAATGCCACGCTTCTGC‐3′ | ||
| Ins2 | Forward: 5′‐GTGACCAGCTACAGTCGGAA‐3′ | Rat |
| Reverse: 5′‐GAGCTTCCACCAAGTGAGAAC‐3′ | ||
| NeuroD1 | Forward: 5′‐GCTCCAGGGTTATGAGATCGT‐3′ | Mouse |
| Reverse: 5′‐CATCTGTCCAGCTTGGGGGA‐3′ | ||
| Nkx6‐1 | Forward: 5′‐GCACGCTTGGCCTATTCTCT‐3′ | Mouse |
| Reverse: 5′‐GAAGTCCCCTTGAGCCTCTC‐3′ | ||
| Pet1/Fev | Forward: 5′‐AGATCCCGTCGGAGATGGTC‐3′ | Mouse |
| Reverse: 5′‐GGTCGGTGAGCTTGAACTCG‐3′ | ||
| Pdx1 | Forward: 5′‐ACTTAACCTAGGCGTCGCAC‐3′ | Mouse |
| Reverse: 5′‐AGCTCAGGGCTGTTTTTCCA‐3′ | ||
| Slc6a4 | Forward: 5′‐GGCGCGAGGGTCGAG‐3′ | Mouse |
| Reverse: 5′‐ATGCTGGTCAGTCAGTG‐3′ | ||
| Tbp | Forward: 5′‐ACCCCTTCACCAATGACTCCTATG‐3′ | Mouse |
| Reverse: 5′‐ATGACTGCAGCAAATCGCTTGG‐3′ | ||
| Tbp | Forward: 5′‐ACAGCCTTCCACCTTATGCT‐3′ | Rat |
| Reverse: 5′‐ GCTGCTGTCTTTGTTGCTCT‐3′ | ||
| Tph2 | Forward: 5′‐TCTACACCCCGGAACCAGAT‐3′ | Mouse |
| Reverse: 5′‐GCAAAGGCCGAACTCGATTG‐3′ |
qPCR products were performed with primer pairs amplifying short fragments for each gene. Primer pairs were custom‐designed to efficiently anneal to homologous nucleotide sequences from mouse.