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. 2020 Jan 2;27:2. doi: 10.1186/s12929-019-0600-3

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — GAG modification is critical for SRGN binding to CD44. CM was harvested from H1299/Mock, H1299/SRGN or H1299/SRGN(S/A) cells, and incubated with CD44-Fc or IgG control for 18 h at 37 °C. Western blot analysis of SRGN in the input proteins and proteins bound to CD44-Fc and IgG using anti-SRGN antibody was shown