Figure 1.

Identification and location of the ctb-1 deletion in MA line 1G. (a) A 499 bp frameshift mtDNA deletion in ctb-1 (Δctb-1) occurred spontaneously in a C. elegans spontaneous mutation accumulation line, 1G, and was detected via whole-genome sequencing [44]. The figure shows the read depth from Illumina whole genome sequencing mapped to the ctb-1 region of the C. elegans mitochondrial genome in line 1G. The read depth inside the deletion is only 4% of the read depth of the sequences immediately flanking the deletion, suggesting that the frequency of the Δctb-1 mitotype is 96% within this MA line. (b) A map of the C. elegans mitochondrial genome (adapted from [48]), illustrating the location, extent and the sequence context of Δctb-1. The protein-coding sequence of the ctb-1 gene spans 1110 bp (protein length 370 aa). (Online version in colour.)