Figure 4. Localization and Phenotype of Pancreas Macrophages.

(A) Light scatter—forward scatter (FSC) and side scatter (SSC)—profile of DAPI^loCD45⁺ cells from a pancreas cell suspension after dissociation in a Ricordi chamber (left). The proportion of these cells expressing the myeloid lineage markers CD14 and CD64 is shown in a representative flow cytometry plot (center) with the compiled percentages of the indicated CD14⁺CD64⁺ subset (right) (± SEM from 12 to 16 donors for each tissue).

(B and C) Expression of tissue macrophage markers CD163 and CD206 (B), as well as MHC class II and CD86 (C), on the DAPI^lo CD45⁺CD64⁺CD14⁺ myeloid cells isolated from pancreas, jejunum, PLN, and spleen. Shown are representative flow cytometry plots (left) with the compiled percentages of the indicated subsets (right) (±SEM from 12 to 16 donors for each tissue). **p < 0.01 as calculated by one-way ANOVA with Dunnett’s multiple comparisons test.

(D) Density of pancreas macrophages in endocrine and exocrine pancreas. Shown is a representative qmIF image with the single-marker CD163 (left) and the corresponding composite image (right) of CD163 (yellow), the ductal marker CK19 (green), and the neuroendocrine marker chromogranin (white). Acinar, ductal, and endocrine areas were defined using inForm software, and the densities of CD163⁺ macrophages were quantified in the indicated pancreatic tissue areas (right) (± SEM from 13 donors). **p < 0.01 as calculated by one-way ANOVA with Sidak’s multiple comparisons test. White bar, 100 µm for scale.

See also Figure S4.