Table 2.

Nitric oxide release, fluorescence measurements, and in vitro anti-proliferative activity

compd	nitric oxide yield (%)a	fluorescencec ± S. D.d (a.u.)	relative fluorescence (%)	IC50 (μM)e
DMSO	0	7.7 ± 0.25	100	-
2	100	9.5 ± 0.13	124	>20f
3	87b	7.9 ± 0.09	103	9.6g
4	96	7.4 ± 0.18	96	>20
5	98	17.0 ± 0.49	221	5.4
6	100b	28.9 ± 1.26	375	2.7
7	100	17.0 ± 0.49	221	6.0
8	100	24.3 ± 0.85	315	3.4
9	100	26.7 ± 0.85	346	4.8
10	99	24.9 ± 0.70	324	1.4

^aNitric oxide yields from the decomposition of the compound (50-100 μL of a 0.1 mM DMSO solution) in the presence of GSH (3.6 mM) in 0.1 M phosphate buffer (3.5 mL) containing 50 μM diethylenetriamine pentaacetic acid (DTPA) at pH 7.4 and 37 °C as measured by chemiluminescence.

^bCalculated based on 4 moles of NO per mole of compound.

^cMean intracellular NO release measured using the NO-sensitive DAF-FM diacetate dye in HL-60 cells measured in arbitrary units (a.u.).

^dStandard deviations of fluorescence measurements (3 independent experiments).

^eThe 50% inhibitory concentrations are reported for activity against proliferation of HL-60 cells.

^fReference 11.

^gReference 9.