Figure 4.

Wnt/β-catenin signaling in primary human EpSCs. (a) Real-time qPCR for β-catenin and c-Myc. (b) Western blot for β-catenin and c-Myc. The relative expression levels were normalized to GAPDH. Comparisons between two groups were determined by the Student's t test; significance level was set at ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001. (c) The identification of β-catenin shRNA. (d) The cell proliferation ability mediated by CAG up to 3 days. (e) Representative images of Transwell migration assay. (f) Representative images of scratch wound healing assay. Original magnification, ×250.