TABLE 2.

Deletion of Mthfd1l does not affect proliferation in MEFs

	% EdU			% PHH3
Media/genotype	WT	Null	p	WT	Null	p
DMEM	19.2 ± 2.7	25.4 ± 3.5	.13	16.3 ± 6.9	19.3 ± 5.1	.48
MEM	27.8 ± 2.0	32.1 ± 4.8	.48	17.4 ± 6.6	16.7 ± 5.6	.94
MEM+F	24.5 ± 1.9	26.6 ± 2.0	.31	15.1 ± 5.7	15.8 ± 4.4	.59

Note: In cultured embryonic fibroblasts, cell cycle analysis was performed using ethynyl-deoxyuridine (EdU) incorporation for labeling cells in S-phase and phosphohistone H3 (PHH3) staining for labeling cells in mitosis. The experiment was conducted in different culture media condition: DMEM, nutrient-rich medium; MEM, nutrient-limited medium; MEM+F, nutrient-limited medium with formate supplementation. p value was calculated by Mann–Whitney U test.