Figure 8.

Ex vivo brains injected with vesicular stomatitis virus (VSV) into NA generate fictive advertisement calls. A, Unilateral injection into NA of recombinant VSV encoding its own glycoprotein [rVSV(VSV-G)]. White arrowheads indicate the midline. Inset, NA expressing Venus 2 at 48 h post-injection. Note labeled axons in the laryngeal nerve at left. B, Reporter gene expression in the somata of vocal motor neurons. C, Fictive advertisement call in response to the application of serotonin elicited from the brain illustrated in A. Modified from Yamaguchi et al. (2018).