Figure 1.

Molecular characterization of the NEXMIF KO mouse. A, NEXMIF expression was observed only in the nuclear fraction of wild-type mouse cortical lysates but not in cytoplasmic or KO animal lysates. B, Immunohistochemistry in P0 mouse cortical brain slices shows NEXMIF signal in the cortical plate from WT animals, with only background signal in brain slices from KO animals. Scale bar, 20 μm. C–F, Cell density in cortex (C,D) or in hippocampus (E,F) was similar in KO and WT animals at P0. UpCP, Upper cortical plate; LoCp, lower cortical plate; IZ, intermediate zone; VZ, ventricular zone; CA1, cornu ammonis area 1; CA3, cornu ammonis area 3; DG, dentate gyrus. The yellow dashed line indicates the pia. Scale bars, 20 μm. Data are represented as average ± SEM. ns, Not significant. For post hoc power analyses, see Figure 1-1.