Skip to main content
. 2020 Jan 2;40(1):237–254. doi: 10.1523/JNEUROSCI.0222-19.2019

Figure 8.

Figure 8.

Altered spine morphology and synaptic protein composition in the NEXMIF KO mice. A, Golgi staining of spines from pyramidal neurons in P90 mouse hippocampus. Scale bar, 3 μm. B, Representative morphologies of the different dendritic protrusion types classified. C, Quantification of protrusion morphologies in WT and KO animals showed an increase in immature morphologies. D, E, Spine density (D) was decreased and spine length (E) increased in KO animals. F, Western blot of synaptic-related proteins from WT and NEXMIF KO hippocampus. G, Quantification of Western blot data from hippocampus showed a reduction in GluA1, GABAα1, gephyrin, no change in synapsin and no change in PSD-95 intensity. H, Western blot of synaptic-related proteins from WT and NEXMIF KO cortex. I, Quantification of Western blot data from cortex showed a reduction in GluA1, GABAα1, gephyrin, no change in synapsin and no change in PSD-95 intensity. J, Immunohistochemistry of KO and WT hippocampal brain slices at P0 shows a clear decrease in total GluA1 staining intensity Scale bars, 100 μm (full picture); 50 μm (enlarged area). Data are represented as average ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. ns, Not significant.