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. 2019 Nov 13;4(1):77–91. doi: 10.1002/hep4.1450

Figure 5.

Figure 5

HSCs carrying the PNPLA3 I148M SNP increase the expression of cytokines, chemokines, and growth factors when co‐cultured in the MPS NASH model. PHH, HK, and HSC co‐cultures were cultured in the MPS for 15 days in HEP‐LEAN, HEP‐FAT, or HEP‐FAT + LPS medium (LPS was added to culture from day 8 onward). Cultures contained either WT HSC or HSC with the PNPLA3 I148M SNP mutation. On day 15, cell culture medium was analyzed by Luminex for the presence of human cytokines, chemokines, and growth factors. Data are presented as means ± SD for 12 independent cultures (3 HSC donors per condition and n = 4 per donor); *P < 0.05. Abbreviations: FGF‐2, Fibroblast Growth Factor 2; G‐CSF, Granulocyte‐Colony Stimulating Factor; GM‐CSF, granulocyte‐macrophage colony‐stimulating factor; IFN, interferon; and PDGF, platelet‐derived growth factor.