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. 2020 Apr 4;105(1):124–135. doi: 10.3324/haematol.2018.212126

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Figure 1. — Production of IL-6 by bone marrow cells and mast cells is enhanced in patients with systemic mastocytosis (SM) in association with the D816V-KIT allelic burden. (A) IL-6 release from bone marrow mononuclear cells isolated from five patients with SM with D816V-KIT bone marrow allelic frequencies of <5 (Patients 4 and 5 in Online Supplementary Table S1) or >5 (Patients 6, 7, and 10). BM aspirates were cultured for 2-4 days and IL-6 released into the culture media was measured by ELISA. Data are the mean±Standard Error of Mean (SEM). (B) Correlation between IL-6 released into the media by BM cells and the percentage of mast cells in those cultures which was determined by flow cytometry. (C) Flow cytometry histograms showing intracellular IL-6 staining in BM mast cells. Mast cells were gated as CD3⁻/CD34⁻/KIT³/FcεRI⁺ within the BM cells of three patients (Patients 1-3 in Online Supplementary Table S1), with D816V-KIT allelic frequencies in the bone marrow also indicated in the figure. Patient 1 had idiopathic anaphylaxis and did not meet criteria for SM but was used as a control. The percentage of IL-6 positive cells within the mast cell population is indicated in the histograms. ISM: indolent SM.