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. 2019 Nov 12;4(1):92–108. doi: 10.1002/hep4.1448

Figure 6.

Figure 6

Hmgb1 ΔIEC mice on the HFCFD have increased SR‐B1 and decreased ApoB48 protein expression in IEC. WT and Hmgb1 ΔIEC mice were fed the CD or HFCFD for 1 week, and IEC were isolated. (A) Western blot analysis for ACAT2, ApoB48, FABP2, MOGAT2, MTP, and immunoprecipitation followed by western blot for SR‐B1 normalized to actin or calnexin. Data are expressed as means ± SEM (n ≥ 3 mice/group). † P < 0.05 for HFCFD versus CD; *P < 0.05 for Hmgb1 ΔIEC versus WT mice. (B) Proposed mechanism by which HMGB1 is delaying and/or reducing CM release: In Hmgb1ΔIEC mice fed the HFCFD, SR‐B1 is increased and ApoB48 expression is significantly decreased, whereas Mttp (encoding MTP) is only slightly down‐regulated. This contributes to increased absorption of CHO and hinders the packaging of TG and CHO to form CM, thereby diminishing CM efflux. This results in lower lipid classes in serum and hence less hepatosteatosis. Abbreviations: IP, immunoprecipitation; MAG, monoacylglycerol.