FIG. 3.
CpG 2395 decreases B cell proliferation, while DNA 2395 and TNA 2395 do not alter B cell proliferation. Ramos cells were plated at a density of 2 × 105 cells in 12-well plates and stimulated with the indicated ligands at the indicated concentrations. After 24 (A) or 72 (B, C) hours, cells were collected and counted using a hemacytometer. Cell counts were confirmed using a flow cytometer to ensure counting accuracy. Cell counts relative to the untreated control are shown. Experiments were repeated 3 times.