Fig. 2.

Intestinal expression levels of the eCBome receptors Cnr1 (A), Gpr18 (B), Gpr55 (C), and Pparα (D) in 4- and 13-week-old CR and GF mice and in 13-week-old male GF mice gavaged with sterile PBS (SHAM) or fecal microbiota (FMT). The mRNA expression levels were measured by qPCR array in duodenum, jejunum, ileum, and colon for all experimental groups and are expressed relative to CR mice for each age within each tissue. Values are mean ± SEM of n = 5–10 mice. Bars marked with different letters were significantly different at the P ≤ 0.05 level. E, F: Gpr55 gene expression in 7-week-old CR, GF, and FMT Swiss Webster mice (E) and control (CR) or antibiotic-treated (Ab) C57BL/6J mice (F).