Fig. 1.

Cyp2a12 and Cyp2c70 are the genes responsible for hepatic BA 7α-hydroxylation and CDCA 6β-hydroxylation. A: Comparison of BA metabolism between human and mouse. B: Orthology among mouse, rat, and human CYP genes. C: Species difference, sexual dimorphism, and tissue distribution of microsomal BA 7α-hydroxylase and CDCA 6β-hydroxylase activities. GDCA, glycodeoxycholic acid. Data are represented as means of duplicate assay. D: Methods for narrowing down candidate mouse genes encoding BA 7α-hydroxylase and CDCA 6β-hydroxylase using orthology, tissue distribution, and sexual dimorphism data of Cyp. E: LC-selected reaction monitoring chromatograms (24) of taurocholic acid (TCA) and TCDCA in extracts obtained from the incubation mixture of recombinant rat CYP2A1, NADPH generating system, and TDCA or TLCA. Q10