Fig. 4.

BA pool size and cholesterol metabolism are markedly affected in 2c70KO and DKO mice. A total of 44 mice (n = 6, 7, 4, 10, 5, 4, 4, and 4 for male WT, 2a12KO, 2c70KO, DKO, female WT, 2a12KO, 2c70KO, and DKO, respectively) were euthanized at 11.1 ± 0.8 (mean ± SD) weeks of age, and serum, individual organs, and feces were analyzed. A: The total amount of BAs in the enterohepatic circulation was calculated by adding total BAs in the liver, gallbladder, and small intestine. B: BA concentrations in feces. C: Proportion (mole percent) of BAs, cholesterol, and phospholipids in gallbladder bile. D–G: Total cholesterol concentrations in serum (D) and the liver (E) and total triglyceride concentrations in serum (F) and the liver (G). Each column and error bar represents the mean and SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 were considered significantly different by the Tukey-Kramer test.