KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial Strains
E. coli C41(λDE3)	Lucigen	60442
Chemicals, Peptides, and Recombinant Proteins
Selenomethionine	Anatrace	S2000
LDAO	Anatrace	D360S
DDM	Anatrace	T350
C8E4	Anatrace	D310S
Nis-Pur Ni-NTA Resin	Thermo-Fisher	88221
Superdex S200 10/30	GE	17-1043-10
Critical Commercial Assays
MemGold	Molecular Dimensions	MD-139
Additive Screen	Hampton Research	HR2–428
Deposited Data
E. coli BcsC2	This paper	PDB: 6TZK
E. coli PgaA	(Wang etal., 2016)	PDB:4Y25
P. aeruginosa AlgE	(Whitney et al., 2011)	PDB:3RBH
Oligonucleotides
EcBCS_PelB_10His_TEV_C2_F GAAAACCTGTACTTCCAGAGTGACAAGTTAATACACACGAGC	IDT	N/A
Unverisal_PelB_10His_TEV_R TGGAAGTACAGGTTTTCATGATGGTGATGATGATGGTGATGGTGG	IDT	N/A
Recombinant DNA
pET20_PelB_8His_BcsC	This paper	N/A
pET20_PelB_10His_TEV_BcsC2	This paper	N/A
Software and Algorithms
HKL3000	(Minor et al, 2006)	https://sbgrid.org/
Phenix 1.9	(Adams et al., 2010)	https://sbgrid.org/
Coot	(Emsley and Cowtan 2004)	https://sbgrid.org/
Pymol 2.2	Schrodinger, LLC	https://sbgrid.org/
CCP4 7.0	Winn et al., 2011	https://sbgrid.org/
Other