Fig. 6. Naive CD8⁺ T cell preconditioning occurs in lymph nodes, but not spleens.

(A) C57BL/6 mice were treated with FTY720 to block lymphocyte egress from all lymphoid tissues. After 4 weeks of continued treatment, naive CD8⁺ T cells in LNs and spleen were analyzed for CD103 expression. Data are means and replicates and representative of six independent experiments. (B) CD103 expression in naive CD8⁺ T cells in spleens and thymi of WT and Lta^−/− mice. Data are means and replicates and representative of two independent experiments. (C) Frequency of CD69⁺ CD103⁺ eT_RM cells in skin 4 weeks after ‘prime and pull’ immune challenge through i.v. injection with OVA-expressing Listeria monocytogenes to produce a circulating T_EFF cell pool (“prime”) followed by DNFB⁺ treatment of ear skin (“pull”) of WT an Lta^−/− mice. Data are means and replicates and representative of four independent experiments. (D) CD103 expression of naive CD8⁺ T cells from αV-ΔDC donors in indicated SLOs of continuously FTY720-treated WT hosts 4 weeks following adoptive transfer. Data are means and replicates and representative of two independent experiments. */**/***/****: p<0.05/p<0.01/p<0.001/p<0.0001 (Two-tailed unpaired Student’s t-tests in (A-C), One-way ANOVA in (D)).