Fig. 7. αVβ8-expressing migratory DCs precondition naive CD8⁺ T cells for epithelial T_RM cell formation in skin.

(A) CD11c^int MHC II^hi migratory DCs in LNs are absent in spleens of C57BL/6 mice. (B) CD103⁺ and CD11b⁺ subsets of migratory DCs as well as CD8⁺ and CD11b⁺ subsets of resident DCs were purified by FACS and analyzed for expression of αV and β8 integrin mRNA by RT-qPCR. Data are means and replicates and representative of two independent experiments. (C) CD11c^int MHC II^hi migratory DCs are absent in LNs of Ccr7^−/− mice. (D) CD103 expression in naive CD8⁺ T cells in pooled LNs and spleen of Ccr7^−/− mice. Data are means and replicates and representative of four independent experiments. (E) 10⁶ naive OT-I T cells were adoptively transferred into Ccr7^−/− or C57BL/6 hosts. CD103 expression on naive OT-I cells from pooled LNs and spleen was analyzed 3 weeks later. Data are means and replicates and representative of two independent experiments. **/****: p<0.01/p<0.0001 (Two-tailed unpaired Student’s t-tests in (D-E)).