Table 3.
mTOR/Akt phosphoprotein cell signaling in 3xTg-AD mice.
| Protein | Phosphorylation site | mPFC | PFC | Acb | AMY | MHPC | LHPC | CA1 | LEC | MEC |
|---|---|---|---|---|---|---|---|---|---|---|
| Akt/PKB | (Ser473) | 111.3 | 91.4 | 90.7 | 95.7 | 113.5 | 103.2 | 81.7 | 68.6 | 81.8 |
| ERK 1/2 | (Thrl85/Tyrl87) | 101.9 | 80.3 | 91.4 | 69.0* | 109.6 | 74.3 | 93.0 | 74.0 | 64.7 |
| GSK3α | (Ser21) | 72.6 | 71.1 | 91.4 | 97.5 | 82.2 | 84.9 | 122.8 | 52.4 | 60.5* |
| GSK3β | (Ser9) | 130.6 | 99.4 | 90.6 | 96.8 | 114.9 | 91.7 | 83.4 | 101.2 | 109.6 |
| IGF1R | (Tyrl135/1136) | 89.7 | 86.2 | 91.5 | 86.7 | 79.7 | 68.2 | 104.7 | 59.7* | 69.4* |
| IR | (Tyrl162/1163) | 105.6 | 113.2 | 81.9 | 86.8 | 98.8 | 91.1 | 92.5 | 54.4* | 80.4 |
| IRS1 | (Ser636) | 94.0 | 88.3 | 82.7 | 106.4 | 80.0 | 66.3* | 88.2 | 56.4 | 64.3* |
| mTOR | (Ser2448) | 102.0 | 86.6 | 82.8 | 74.0 | 84.1 | 85.9 | 76.3* | 68.5 | 82.2 |
| p70S6K | (Thr389/412) | 74.7 | 117.1 | 50.7 | 87.7 | 65.1 | 49.4* | 142.0 | 62.0 | 59.7 |
| PTEN | (Ser380) | 104.6 | 106.1 | 89.1 | 94.7 | 108.3 | 113.6 | 80.3* | 72.9* | 109.1 |
| RPS6 | (Ser235/236) | 96.1 | 93.1 | 81.3 | 93.9 | 87.7 | 83.7 | 103.5 | 60.2 | 73.7* |
| TSC2 | (Ser939) | 104.8 | 117.0 | 99.7 | 119.3 | 115.4 | 78.6 | 81.3 | 94.1 | 101.4 |
This table shows the percent change in relative protein expression of 3xTg-AD alcohol drinking mice compared to saccharin drinking mice at 1-month post alcohol (8-months old). Relative change calculated using β-Tubulin as loading control. All analytes and brain regions were treated as independent samples for analysis. Shaded boxes with * indicate P < 0.05; for one-tailed, t-test.
Abbreviations: protein kinase B (Akt/PKB), mitogen-activated protein kinase/extracellular signal-regulated kinases pathway (ERK 1/2), glycogen synthase kinase 3α (GSK3α), glycogen synthase kinase 3β (GSK3β), insulin-like growth factor 1 receptor (IGF1R), insulin receptor (IR), insulin receptor substrate 1 (IRS1), mammalian target of rapamycin (mTOR), ribosomal protein S6 kinase β−1 (p70S6K), phosphatase and tensin homolog (PTEN), ribosomal protein S6 (RPS6), mTOR complex 2 (TSC2).