Fig. 2.

Immunotherapy increases CD4⁺ and CD8⁺ TIL. a Representative flow plots and b corresponding graph of the frequency of tumor-infiltrating CD4⁺, CD8⁺, NK, and Tregs in mice treated with PBS control (open circle) or ICB (closed circle). Mice received bilateral subcutaneous injections of 1 × 10⁶ B16-F0 melanoma cells and were treated with ICB or PBS on days 8, 11, and 14 post-tumor injection (n = 15). Tumors were harvest on day 16 and analyzed by flow cytometry for immune cell frequency and c CD8⁺ TIL visualized by IHC. P values are indicated above each treatment group