Fig. 3.

Checkpoint blockade increases TIL effector functions. Mice received bilateral subcutaneous injections of 1 × 10⁶ B16-F0 melanoma cells and were treated with immune checkpoint blockade or PBS control on days 8, 11, and 14 post-tumor injection. Tumors were harvest on day 16 and analyzed by flow cytometry for effector molecule production. a Representative flow plots and b corresponding graph of the frequency of IFNγ and TNF producing tumor-infiltrating CD8⁺, CD4⁺, and NK cells from mice treated with PBS control (open circle) or ICB (closed circle) after ex vivo restimulation. c MFI of IFNγ for CD8⁺ and CD4⁺ TIL. d Frequency of Granzyme B producing CD8⁺ TIL (n = 14). For graphs b and c, n = 17 from five independent experiments. For graph c, n = 14 from four independent experiments. P values are indicated above each treatment group