Fig. 4.

Immunotherapy induces CD8⁺ TIL reinvigoration. Mice with established B16 tumors were treated with ICB or PBS control on days 8, 11, and 14 post-tumor injection. Tumors were harvest on day 16 and analyzed by flow cytometry. a Representative flow plots of CD8⁺ TIL from mice treated with PBS control or ICB. Red quadrant and inset numbers in the top panels are the percentage of Eomes⁺ PD1⁺ CD8⁺ TIL. Red contour in the bottom panels are reinvigorated (Eomes⁺ PD-1⁺ T-bet⁺ Ki67⁺) CD8⁺ TIL taken from the red quadrant above. Inset numbers are the percentage of reinvigorated CD8⁺ TIL. Pooled data for exhausted (b) and reinvigorated (c) CD8⁺ TIL (n = 17) are displayed from five independent experiments. P values are indicated above each treatment group