Fig. 5.

Increased ratio of insoluble Tau to Aβ in TREM2 cases. Western blot analysis of insoluble tau in the final supernatant (a–c) and pellet (d–f) of protein extraction. a, d Total tau (17,025, red), b, e phosphorylated tau (PHF1, green), and (c, f) overlay. Quantification of insoluble tau in final supernatant (g) and pellet (j) as well as total insoluble tau (supernatant + pellet, m). Insoluble Aβ42 quantified by ELISA in final supernatant (h), final pellet (k), and total (supernatant + pellet, n). Ratio of insoluble tau and insoluble Aβ42 in final supernatant (i), final pellet (l) and combined total (supernatant + pellet, o). *p < 0.05, unpaired Student’s t test