Fig. 4. Typical flow cytometric plots and different phenotypes of lymphocytes.

Flow cytometry was employed to investigate the differentiation of CD4⁺ lymphocytes. (A and B) The representative flow cytometric plots of CD4⁺CD25⁺Foxp3⁺ Treg and CD4⁺IL-17⁺ Th17 cells, respectively, labelled with the corresponding percentage of CD4⁺ T cells. (C-E) The ratios of Th17/CD4⁺, ratio of Treg/CD4⁺ and ratio of Treg/Th17 respectively. Data are presented as mean ± SD, n = 6. *P < 0.05, ^#P > 0.05. Group 1, control group; Group 2, model group; Group 3, MSC-exosomes-treated group; Group 4, MSC-exosomes^miR-223-3p-treated group; Group 5, MSC-exosomes^{miR-223-3p(i)}-treated group; Group 6, drug-treated group.