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. 2019 Mar 13;70(6):1755–1766. doi: 10.1093/jxb/erz058

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Fig. 4. — The decline in electron transport rate is diagnostic of TPU limitation. Combined gas exchange and fluorescence data in A/C_i curves of Nicotiana benthamiana at varying light intensity and 35 °C. At low CO₂, plants are limited by Rubisco activity (C limitation, red), characterized by a sharp upwards slope of both A and ETR with increasing CO₂. When light is insufficient, plants will be limited by the rate of RuBP regeneration (J limitation, green), characterized by a flat slope of the ETR with increasing CO₂. Only when the plant has ample CO₂ and electron transport will TPU limitation (P, yellow) be seen, characterized by a decline in ETR with increasing CO₂. ETR is calculated from fluorescence-derived Φ_PSII. Light intensity (µmol m⁻² s⁻¹): , 250; , 400; , 550; , 750; , 1000; 1500.

Inline graphic — The decline in electron transport rate is diagnostic of TPU limitation. Combined gas exchange and fluorescence data in A/C_i curves of Nicotiana benthamiana at varying light intensity and 35 °C. At low CO₂, plants are limited by Rubisco activity (C limitation, red), characterized by a sharp upwards slope of both A and ETR with increasing CO₂. When light is insufficient, plants will be limited by the rate of RuBP regeneration (J limitation, green), characterized by a flat slope of the ETR with increasing CO₂. Only when the plant has ample CO₂ and electron transport will TPU limitation (P, yellow) be seen, characterized by a decline in ETR with increasing CO₂. ETR is calculated from fluorescence-derived Φ_PSII. Light intensity (µmol m⁻² s⁻¹): , 250; , 400; , 550; , 750; , 1000; 1500.