KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-FLAG Peroxidase (clone M2) | MilliporeSigma | Cat# A8592; RRID:AB_439702 |
| Mouse monoclonal antibody to PdpA | BEI Resources | Cat# NR-3197 |
| Goat anti-mouse IgG peroxidase conjugate | Pierce-Thermo | Cat# 32230 |
| Goat anti-rabbit IgG peroxidase conjugate | Bio-Rad | Cat# 170-6515 |
| Rabbit polyclonal antibody to IglC | Clemens et al., 2015 | N/A |
| Bacterial and Virus Strains | ||
| E. coli NEB 5-alpha | New England BioLabs | Cat# C2987H |
| E. coli NEB 5-alpha F’ Iq | New England BioLabs | Cat# C2992H |
| E. coli Rosetta 2(DE3) | MilliporeSigma | Cat# 71400 |
| E. coli BTH101 | Euromedex | Cat# EUB001 |
| F. novicida IglAΔ | Clemens et al., 2015 | Fn IglAΔ |
| F. novicida PdpAΔN | This Study | Fn PdpAΔN |
| F. novicida PdpAΔC | This Study | Fn PdpAΔC |
| F. novicida PdpA::GSSG | This Study | Fn PdpA::GSSG |
| F. novicida PdpA-FLAG | This Study | Fn PdpA-FLAG |
| F. novicida FLAG-VgrG | Clemens et al., 2015 | FV, Fn-FLAG-VgrG |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Adenosine triphosphate | Sigma-Aldrich | Cat# A-6419 |
| 3X FLAG peptide | Sigma-Aldrich | Cat# F4799-25MG |
| N-ethylmaleimide | Sigma-Aldrich | Cat# E3876 |
| Phenylmethylsulfonyl fluoride | Sigma-Aldrich | Cat# P-7626 |
| Benzonase Nuclease | VWR | Cat# EM71206-3 |
| Tween 20 | Fisher Chemical | Cat# BP337-500 |
| Critical Commercial Assays | ||
| Tetramethyl benzidine substrate kit | Thermo Fisher Scientific | Cat# 34021 |
| Deposited Data | ||
| P. aeruginosa PA0091 VgrG1 X-ray structure | PDB | PDB 4MTK |
| T4 phage (gp27-gp5)3 X-ray structure | PDB (Koshyiama et. al, 2008) | PDB 2Z6B |
| P. aeruginosa VgrG1 X-ray structure | PDB (Quentin et al., 2018) | PDB 6H3L |
| F. novicida central spike complex, lidded atomic model | This Study | PDB 6U9F |
| F. novicida central spike complex, half-lidded atomic model | This Study | PDB 6U9G |
| F. novicida central spike complex, lidless atomic model | This Study | PDB 6U9E |
| F. novicida central spike complex, lidded cryo-EM density map | This Study | EMD-20696 |
| F. novicida central spike complex, half-lidded cryo-EM density map | This Study | EMD-20698 |
| F. novicida central spike complex, lidless cryo-EM density map | This Study | EMD-20695 |
| Experimental Models: Cell Lines | ||
| THP-1 Cells | ATCC | NIH-ARP Cat# 9942-142, RRID:CVCL_0006 |
| Experimental Models: Organisms/Strains | ||
| Francisella novicida Utah 112 | Karl Klose, University of Texas San Antonio | WT |
| Recombinant DNA | ||
| pET28a | MilliporeSigma | Cat# 69864 |
| pET28-His18-PdpA | This Study | N/A |
| pET28-His18-PdpA::GSSG | This Study | N/A |
| pET28-3xFLAG-VgrG G164R | This Study | N/A |
| pKNT25 | Euromedex | Cat# EUP-25N |
| pKT25 | Euromedex | Cat# EUP-25C |
| pKT25-zip | Euromedex | Cat# EUP-25Z |
| pUT18 | Euromedex | Cat# EUP-18N |
| pUT18C | Euromedex | Cat# EUP-18C |
| pUT18C-zip | Euromedex | Cat# EUP-18Z |
| pKNT25-IglA | This Study | N/A |
| pKNT25-VgrG | This Study | N/A |
| pKNT25-IglC | This Study | N/A |
| pKT25-IglC | This Study | N/A |
| pKT25-VgrG | This Study | N/A |
| pUT18-IglB | This Study | N/A |
| pUT18-PdpA2-175 | This Study | N/A |
| pUT18-PdpA172-621 | This Study | N/A |
| pUT18-PdpA762-820 | This Study | N/A |
| pUT18C-PdpA2-175 | This Study | N/A |
| pUT18C-PdpA172-621 | This Study | N/A |
| pUT18C-PdpA762-820 | This Study | N/A |
| pMP590 | Martin Pavelka, University of Rochester | N/A |
| pMP590-pdpAΔ2-175-updn | This Study | N/A |
| pMP590-pdpAΔ762-820-updn | This Study | N/A |
| pMP590-pdpA::GSSG-updn | This Study | N/A |
| pMP590-pdpA-FLAG-updn | This Study | N/A |
| Software and Algorithms | ||
| Leginon | Carragher et al., 2000 | http://nramm.nysbc.org/downloads |
| MotionCor2 | Zheng et al., 2017 | http://emcore.ucsf.edu/ucsf-motioncor2 |
| CTFFIND 4.18 | Rohou and Grigorieff, 2015 | http://grigoriefflab.janelia.org/ctf |
| Gautomatch | Dr. Zhang, MRC Laboratory of Molecular Biology | http://www.mrc-lmb.cam.ac.uk/kzhang |
| Relion-2.1 | Fernandez-Leiro and Scheres, 2017 | http://www3.mrc-lmb.cam.ac.uk/relion |
| CryoSparc | Punjani et al., 2017 | http://cryosparc.com/ |
| PHENIX | Adams et al., 2010 | http://www.phenixonline.org/ |
| Dali Server | Holm and Rosenstrom, 2010 | http://ekhidna2.biocenter.helsinki.fi/dali/ |
| COOT | Emsley et al., 2010 | http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/. |
| PDBePISA Assembly | Krissinel and Henrick, 2007 | http://www.ebi.ac.uk/pdbe/pisa/ |
| PsiPred server | McGuffin et al., 2000 | http://bioinf.cs.ucl.ac.uk/psipred/ |
| I-Tasser server | Yang et al., 2015 | http://zhanglab.ccmb.med.umich.edu/I-TASSER/ |
| JPred4 | Drozdetskiy et al., 2015 | http://www.compbio.dundee.ac.uk/jpred4/ |
| MolProbity | Chen et al., 2010 | http://molprobity.biochem.duke.edu/ |
| UCSF Chimera | Pettersen et al., 2004 | http://www.cgl.ucsf.edu/chimera/download.html |
| Other | ||
| SYPRO Ruby | Thermo Fisher Scientific | Cat# S11791 |
| Anti-FLAG M2 affinity gel | MilliporeSigma | Cat# A2220; RRID: AB_10063035 |
| Ni-NTA Agarose affinity gel | Qiagen | Cat# 30210 |
| Trypticase soy broth | BD Difco | Cat# 211768 |
| Protease Inhibitor Cocktail Set III, EDTA-Free | MilliporeSigma | Cat# 539134 |
| Sephacryl S400HR gel filtration resin | Pharmacia | Cat# 17-0609-01 |