Figure 2.

Schematic view of (A) preparation of conditioned media and (B) Migration assay in co-culture system. (A) Cells were seeded at day 1, and the media were replaced with SFM at day 2. The media were replaced again with SFM at day 3 and treated with or without AuNPs for another 2 days. The media were collected, centrifuged to remove cell debris and AuNPs, and stored at −80C° or used freshly. (B) The migration-inducing cells were seeded on outwell, treated with or without AuNPs in SFM for 36h, and then co-cultured with ECs seeded to the inserts for 16 h. ECs migrated to the outside of the insert were then evaluated.