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| Mutation | Forward primer | Reverse primer |
|---|---|---|
| D61A | CAGAAAGTTATCACTAGAGCTAAGAATAATGTCAATGTGG | CCACATTGACATTATTCTTAGCTCTAGTGATAACTTTCTG |
| K89A | CACTATGAGTACACGGCGAAATTGAACAATTCTATC | GATAGAATTGTTCAATTTCGCCGTGTACTCATAGTG |
| S181A | GAGGGTCAGAAGGTTGCTGTCGATTACATGCTA | TAGCATGTAATCGACAGCAACCTTCTGACCCTC |
