Fig. 2.

Evaluation of methods to inactivate TtLPMO9E by quantification of gluconic acid. 50 g L⁻¹ Avicel were incubated with 2 mM ascorbic acid and 1 µM TtLPMO9E in 50 mM citrate buffer pH 6 for 6 h at 50 °C. Prior to the incubation, the samples were treated with either, 20 min incubation at 100 °C, 20 min incubation at 100 °C + 1 mM EDTA, 1 mM EDTA, or 50 mM NaOH. A positive control, not treated with NaOH, EDTA, or incubation at 100 °C, was included. Gluconic acid was quantified by HPAEC-PAD (black bars) and by the UV-based gluconic acid kit (grey bars). All incubations were performed in triplicate and the standard deviations of the samples are represented by error bars