Fig. 3.

Quantification of the soluble and insoluble products from microcrystalline cellulose and pretreated wheat straw (PWS). 50 g L⁻¹ Avicel or 20 g L⁻¹ PWS incubated with 2 mM ascorbic acid and 1 µM TtLPMO9E in 50 mM citrate buffer pH 6 at 50 °C from 0 to 24 h. a, Supernatant of Avicel incubated with TtLPMO9E and ascorbic acid incubated with 5 U mL⁻¹A. niger β-glucosidase. b, Pellet of Avicel incubated with TtLPMO9E and ascorbic acid incubated with 0.5 g L⁻¹ T. longibrachiatum CBHI and 5 U mL⁻¹A. niger β-glucosidase. c, Supernatant of PWS incubated with TtLPMO9E and ascorbic acid incubated with 5 U mL⁻¹A. niger β-glucosidase. d, Pellet of PWS incubated with TtLPMO9E and ascorbic acid incubated with 0.5 g L⁻¹ T. longibrachiatum CBHI and 5 U mL⁻¹A. niger β-glucosidase. Gluconic acid detected after 0 h of incubation was subtracted from the results. Gluconic acid was quantified by HPAEC-PAD (black bars) and by the UV-based gluconic acid kit (grey bars). All results are averages of triplicate measurements. The standard deviations of the samples are represented by error bars. e and f, Correlation between the quantification results measured by HPAEC-PAD (y-axis) and gluconic acid kit (x-axis) from a–d. The equation for the best linear regression (slope = 0.9) and the coefficient of determination, r² (r² = 0.98) is shown. f: Enlargement of the correlation at gluconic acid concentrations from 0 to 50 mg L⁻¹. For clarity, standard deviations are not shown