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. 2019 Dec 26;7(24):4414–4419. doi: 10.12998/wjcc.v7.i24.4414

Table 2.

Precipitation with polyethylene glycol

AST origin AST DIL1/2 AST PEG PPA (%) AST recovery (%)
Patient 71.8 38.9 1.4 96.4 3.60
Control 1 229 121.7 49.8 59.1 40.9
Control 2 68 31 17.7 42.9 57.1

Polyethylene glycol precipitation (PEG): 25% PEG centrifuged at 3000 rpm of two control serum samples and samples from the patient with macro-aspartate aminotransferase (AST). AST origin: AST value of the original serum; AST diluted 1/2 (DIL1/2): AST value of serum DIL1/2 with phosphate-buffered saline; AST PEG: AST value after PEG precipitation. PEG-precipitable activity (PPA) = 100 × [(AST DIL1/2−AST PEG)/AST DIL1/2]; AST recovery = 100 × (AST PEG/AST DIL1/2).