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. 2020 Jan 2;11:63. doi: 10.1038/s41467-019-13710-4

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020

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Fig. 4 — a–c P45 whole-mount utricles from controls (a, a′) and Cyp26b1 cKO (b, b′) immunolabeled with anti-Tuj1 (green) and anti-calbindin (magenta) antibodies. a, b Maximum intensity projection of the entire utricle. a′, c Enlarged, single plane image of the rectangular region in control striola (a), showing the presence of large number of double (white circles, 42.6 ± 1.8 double calyces/utricle) and triple (cyan circles, 10.0 ± 2.0 triple/utricle, n = 3 utricles) calyces at the cell body level. b′, c Fewer double calyces (white circles, 13.6 ± 2.8 double calyces/utricle, P = 0.001, unpaired t test) and no triple calyx (0.6 ± 0.6 triple calyces/utricle, P = 0.006, n = 3) are found in the corresponding region of Cyp26b1 cKO mutants. Scale bars: 200 μm for a, b; 30 μm for a′, b′. **P < 0.01. A, anterior; L, lateral. Error bars: SEM.